Among those who played the game against Bunyore include forward Valerie Nekesa, Charity Midewa and skipper Jane Hato.
Kenya Women Premier League debutants Madira Soccer Assasins tasted their first defeat in the league after bowing 1-0 to Bunyore Starlets in a rescheduled match played on Wednesday at the Ebusakami School Grounds.
Madira who made history after clinching a ticket to the top flight at the end of last season despite majority of their players being students had played two matches in the competition prior to the Wednesday duel.
They had won once and drawn the other fixture, placing eighth on the log with four points, twelve behind leaders Vihiga United who are also unbeaten so are the Kenya Police Bullets.
However, Madira's bid to extend their unbeaten run suffered a blow after going down to Bunyore who shot to the second slot following the win.
Airin Madalina who secured a call up to the Harambee Starlets squad for the forthcoming Africa Cup of Nations qualifier double against Botswana struck the winning goal in the 42nd minute.
Madira fielded a number of players who were part of the Rising Starlets team that unsuccessfully sought to punch a ticket to the World Cup after losing 6-2 on aggregate to Cameroon in round three.
Among those who played the game against Bunyore include forward Valerie Nekesa, Charity Midewa and skipper Jane Hato.
Madira have a chance to move to the top three should they win the three games in hand.
Meanwhile, the Kenya Police Bullets team annihilated Makolanders 7-1 in a friendly game played at the Police Pavillion Grounds in South C on Wednesday.
Goals from Puren Alukwe, Lydia Waganda, Winnie Gwatenda, Lucy Kwekwe and a brace from Chris Kach got the job done for Police Bullets.
Police played newcomers Kibera Soccer to a one-all draw in their previous league assignment.