Mathare beat Kajiado FC 2-1 at the Ildamat Grounds to move five points clear at the summit.
Rainbow who are in their maiden season in the top flight started the season positively but have been unable to maintain the positive run after suffering back to back defeats.
Zoo were relegated from the second-tier last year by the FKF Caretaker Committee. The club had initially been kicked out of the top flight over match fixing allegations.
Seal were hoping to beat Silibwet and move to seven points within the reach of log leaders Shabana but the Murang'a based club had to contend with a point after failing to break the stubborn defence m